ACTION OF CP-8 ON CA2+-CHANNELS OF RAT BRAIN SYNAPTOSOMES

Section: Articles Published Date: 2023-03-20 Pages: 96-100 Issue: VITAL ISSUES OF MODERN SCIENTIFIC RESEARCH

Authors

  • Khoshimov N.N. Institute Of Biophysics And Biochemistry At The National University Of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
  • Nasirov K.E. Institute Of Biophysics And Biochemistry At The National University Of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
  • Dedaboev J.I. Institute Of Biophysics And Biochemistry At The National University Of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
  • Kozokov I.B. National University Of Uzbekistan, Tashkent, Uzbekistan
  • Akhtamov M.A. National University Of Uzbekistan, Tashkent, Uzbekistan
  • Khodjiev S.S. National University Of Uzbekistan, Tashkent, Uzbekistan
  • Abdurakhmonov Zh.A Institute Of Bioorganic Chemistry Named After Academician A.S.Sadikov, Tashkent, Uzbekistan
  • Mukhtorov A.A. Namangan State University, Uzbekistan
  • Mamadaminov R.R. Namangan State University, Uzbekistan
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Abstract

In the study, rat brain synaptosomes were used, which are an adequate and convenient model for studying presynaptic processes. L Glutamate activity was judged by the change in the intensity of the fluorescent signal, by the change in the cytoplasmic levels of free calcium [Ca2+]in. The study was conducted according to the Weiler method. Synaptosomes were isolated from rat brain using two-stage centrifugation. The entire isolation procedure was carried out at 4°C. To measure the amount of cytosolic Ca2+ synaptosomes were calculated according to the Grinkevich equation. An increase in the concentration of [Ca2+]in caused by L glutamate, primarily due to the activation of membrane permeability, the movement of Ca2+ into the cell, and the release of Ca2+ from intracellular depots. PC-8 competes with L glutamate for the glutamate binding site of NMDA-receptors.

Keywords

NMDA-receptors, synaptosomes, L glutamate