ACTION OF CP-8 ON CA2+-CHANNELS OF RAT BRAIN SYNAPTOSOMES

Authors

  • Khoshimov N.N. Institute Of Biophysics And Biochemistry At The National University Of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
  • Nasirov K.E. Institute Of Biophysics And Biochemistry At The National University Of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
  • Dedaboev J.I. Institute Of Biophysics And Biochemistry At The National University Of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
  • Kozokov I.B. National University Of Uzbekistan, Tashkent, Uzbekistan
  • Akhtamov M.A. National University Of Uzbekistan, Tashkent, Uzbekistan
  • Khodjiev S.S. National University Of Uzbekistan, Tashkent, Uzbekistan
  • Abdurakhmonov Zh.A Institute Of Bioorganic Chemistry Named After Academician A.S.Sadikov, Tashkent, Uzbekistan
  • Mukhtorov A.A. Namangan State University, Uzbekistan
  • Mamadaminov R.R. Namangan State University, Uzbekistan

Keywords:

NMDA-receptors, synaptosomes, L glutamate

Abstract

In the study, rat brain synaptosomes were used, which are an adequate and convenient model for studying presynaptic processes. L Glutamate activity was judged by the change in the intensity of the fluorescent signal, by the change in the cytoplasmic levels of free calcium [Ca2+]in. The study was conducted according to the Weiler method. Synaptosomes were isolated from rat brain using two-stage centrifugation. The entire isolation procedure was carried out at 4°C. To measure the amount of cytosolic Ca2+ synaptosomes were calculated according to the Grinkevich equation. An increase in the concentration of [Ca2+]in caused by L glutamate, primarily due to the activation of membrane permeability, the movement of Ca2+ into the cell, and the release of Ca2+ from intracellular depots. PC-8 competes with L glutamate for the glutamate binding site of NMDA-receptors.

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References

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Alisher A. Mukhtorov, Rahmatjon R. Mamadaminov, Nozim N. Khoshimov, Kabil E. Nasirov, Rakhmatilla N. Rakhimov, Lutpillaev X. Gaybullo. Regulation of transport of Ca2+ NMDA-receptors in rat brain synaptosomes under the influence of polyphenols. European Journal of Medicine. 2022. 10(1): 3-11.

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Published

2023-03-20

How to Cite

Khoshimov N.N., Nasirov K.E., Dedaboev J.I., Kozokov I.B., Akhtamov M.A., Khodjiev S.S., Abdurakhmonov Zh.A, Mukhtorov A.A., & Mamadaminov R.R. (2023). ACTION OF CP-8 ON CA2+-CHANNELS OF RAT BRAIN SYNAPTOSOMES. International Scientific and Current Research Conferences, 1(01), 96–100. Retrieved from https://orientalpublication.com/index.php/iscrc/article/view/902

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VITAL ISSUES OF MODERN SCIENTIFIC RESEARCH

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Articles

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Copyright (c) 2023 Khoshimov N.N., Nasirov K.E., Dedaboev J.I., Kozokov I.B., Akhtamov M.A., Khodjiev S.S., Abdurakhmonov Zh.A, Mukhtorov A.A., Mamadaminov R.R.

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